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建立了测定药物中盐酸士的宁的流动注射化学发光分析方法。盐酸士的宁能够显著抑制鲁米诺-Ag(Ⅲ)配合物体系的化学发光作用,且抑制强度与药物的浓度在一定范围内呈良好的线性关系。采用正交设计优化实验条件后盐酸士的宁的最低检出限为2.5×10~(-7)mol/L。线性范围为5.0×10~(-7)~1×10~(-5)mol/L和1.0×10~(-5)~1×10~(-4)mol/L,回归方程分别为△I=68c+16,相关系数r=0.997;△I=233c+518,相关系数r=0.990。对1×10~(-5)mol/L的盐酸士的宁连续进行平行测定11次,相对标准偏差为3.4%,加标回收率在85.4%~101.2%之间。并且应用高效液相色谱法对样品进行了验证,实验结果与化学发光法相符。
A flow injection chemiluminescence method for the determination of strychnine hydrochloride was established. Stanserine hydrochloride can significantly inhibit the chemiluminescence of luminol-Ag (Ⅲ) complex system, and the inhibitory strength and drug concentration showed a good linear relationship within a certain range. The minimum detection limit of strychnine hydrochloride after orthogonal design was 2.5 × 10 -7 mol / L. The linear range was from 5.0 × 10 -7 to 1 × 10 -5 mol / L and 1.0 × 10 -5 to 1 × 10 -4 mol / L respectively. The regression equations were △ I = 68c + 16, the correlation coefficient r = 0.997; △ I = 233c + 518, the correlation coefficient r = 0.990. The parallel determination of 1 × 10 ~ (-5) mol / L of strychnine 11 consecutive times, the relative standard deviation of 3.4%, the recovery was 85.4% to 101.2%. The samples were verified by HPLC. The experimental results are consistent with the chemiluminescence method.