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利用14个SSR标记构建了河南省2015年之前选育并审定的90个花生品种的DNA指纹图谱,用14个SSR标记产生的95个多态性位点可将90个花生品种完全区分开,其中84个品种间有≥2个位点的差异,在剩余的3对品种中,每对仅有1个差异位点。聚类分析结果表明,在遗传相似系数0.98处,90个花生品种被聚集成88类,有2对品种分别聚集在一起,是由于它们每一个品种分别以另一个品种作亲本选育而成,仅有1个差异SSR位点,表明所构建的指纹图谱是有效的。以遗传相似系数0.95为划分标准,有74.4%的品种具有特异性,与其他作物相比,河南省育成花生品种总体上亲缘关系相对较近。根据60个SSR标记的群体结构分析,90个花生品种可以分为3个亚群,与根据分枝开花习性和荚果类型的分类相吻合,亚群划分情况与聚类分析结果基本一致。
Fourteen SSR markers were used to construct the DNA fingerprinting of 90 peanut varieties selected and validated in Henan Province in 2015. 95 polymorphic loci produced by 14 SSR markers could completely distinguish 90 peanut varieties, There were more than 2 loci among 84 varieties, and there was only 1 locus in each of the remaining 3 pairs. The results of cluster analysis showed that at the genetic similarity coefficient of 0.98, 90 peanut varieties were clustered into 88 categories, and 2 pairs of cultivars were clustered together because each of them was bred by another variety as its parent. Only one difference SSR locus indicates that the constructed fingerprint is valid. With genetic similarity coefficient of 0.95 as the standard, 74.4% of the cultivars were specific. Compared with other crops, cultivars of cultivated peanut in Henan Province were relatively close in general. According to the analysis of population structure of 60 SSR markers, 90 peanut varieties could be divided into 3 sub-groups, which were consistent with the classification based on the flowering habits and pod types. The sub-grouping was basically the same as the cluster analysis.