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AIM:To investigate the effect of c9,t11-conjugated linoleicacid (c9,t11-CLA) on the adhesion of human gastriccarcinoma cell line (SGC-7901).METHODS:SGC-7901 cells were at first treated withdifferent concentrations (25,50,100,200μmol/L) of c9,t11-CLA and 1mL/L ethanol (as a negative control) for 24h.Using adhesion assay and Western blot,we investigatedthe ability of SGC-7901 cells to adhere to intracellular matrixand examined the expression of E-cadherin (ECD),α-catenin,intercellular adhesion molecule 1 (ICAM-1) and vascular celladhesion molecule 1 (VCAM-1) in these cells.RESULTS:The attachment rate to laminin of SGC-7901cells treated with different concentrations of c9,t11-CLA (0,25,50,100,and 200μmol/L) was 100.0±3.3,95.7±4.0,89.2±4.6,87.9±6.1,and 65.9±5.8,respectively.Theattachment rate to fibronectin was 100.0±4.7,96.8±3.8,94.5±4.1,76.5±4.3,and 61.8±4.8,respectively.Theattachment rate to Matrigel was 99.9±6.6,91.4±6.8,85.5±7.4,79.3±5.6,and 69.6±5.1,respectively.Besides,c9,t11-CLA could increase the level of ECD and α-catenin,and decrease the level of ICAM-1 and VCAM-1 in SGC-7901 cells.CONCLUSION:cg,t11-CLA can reduce the adhesion ofhuman gastric carcinoma cells to laminin,fibronectin andMatrigel.c9,t11-CLA can increase the level of ECD and α-catenin,and decrease the level of ICAM-1 and VCAM-1 inhuman gastric carcinoma cells.
AIM: To investigate the effect of c9, t11-conjugated linoleic acid (c9, t11-CLA) on the adhesion of human gastric carcinoma cell line (SGC-7901) .METHODS: SGC-7901 cells were at first treated with different concentrations μmol / L) of c9, t11-CLA and 1 mL / L ethanol (as a negative control) for 24h. Facilitating adhesion assay and Western blot, we investigated the ability of SGC-7901 cells to adhere to intracellular matrix and examined the expression of E- The attachment rate to laminin of SGC-7901 cells treated with different concentrations of c9, a-catenin, intercellular adhesion molecule 1 (ICAM-1) and vascular celladhesion molecule 1 (VCAM- t11-CLA (0,25,50,100, and 200 μmol / L) was 100.0 ± 3.3,95.7 ± 4.0,89.2 ± 4.6,87.9 ± 6.1, and 65.9 ± 5.8, respectively. The rate of to fibronectin was 100.0 ± 4.7, 96.8 ± 3.8, 94.5 ± 4.1, 76.5 ± 4.3, and 61.8 ± 4.8, respectively. Attachment rate to Matrigel was 99.9 ± 6.6, 91.4 ± 6.8, 85.5 ± 7.4, 79.3 ± 5.6, and 69.6 ± 5.1, respectively.Bes ides, c9, t11-CLA could increase the level of ECD and α-catenin, and decrease the level of ICAM-1 and VCAM-1 in SGC-7901 cells. CONCLUSION: cg, t11-CLA can reduce the adhesion of human gastric carcinoma cells to laminin, fibronectin and Matrigel.c9, t11-CLA can increase the level of ECD and α-catenin, and decrease the level of ICAM-1 and VCAM-1 inhuman gastric carcinoma cells.