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目的研究麻疹病毒沪-191株在鸡胚成纤维细胞中连续传代的遗传稳定性。方法将麻疹病毒沪-191株原始种子批(P25)在鸡胚成纤维细胞上传代2次(P27)作为主种子批,继续传代3次(P30)作为工作种子批,继续传代至33(P33)和35(P35)代。对P25、P27、P30、P33和P35病毒基因组进行基因序列分析,并对所有代次病毒进行滴度测定。结果麻疹病毒沪-191株在鸡胚成纤维细胞中从25代传至35代,其滴度维持在5.0～5.875 lgCCID50/ml之间;P25、P27、P30、P33、P35 5个代次病毒的全基因组序列未发生任何核苷酸和氨基酸改变。结论麻疹病毒沪-191株在鸡胚成纤维细胞连续传代,病毒滴度均一,基因组序列未发生改变,遗传稳定性良好。
Objective To study the genetic stability of the continuous transmission of measles virus Shanghai-191 strain in chick embryo fibroblasts. Methods The primary seed batch (P25) of Shanghai-191 strain of measles virus was passaged twice on the embryo fibroblasts (P27) as the main seed batch and the third passage (P30) ) And 35 (P35) generations. The gene sequences of the P25, P27, P30, P33 and P35 viral genomes were genotyped and titrated for all generations of viruses. Results The measles virus strain Shanghai-191 was passaged from passage 25 to passage 35 in chick embryo fibroblasts with a titer of 5.0-5.875 lg CCID50 / ml. Five generations of P25, P27, P30, P33 and P35 viruses The whole genome sequence does not have any nucleotide and amino acid changes. Conclusion The measles virus Shanghai-191 strain was continuously passaged in chicken embryo fibroblasts, the virus titer was uniform, the genome sequence did not change, and the genetic stability was good.