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为了观察16Hz、130dB次声对SD大鼠记忆功能的影响及依达拉奉对其脑损害的防治作用。依达拉奉干预组在次声作用前3d开始连续给药(3mg/kg)直至次声作用7d后停止。次声作用7d后,测Y型电迷宫评定大鼠记忆功能,用单链DNA(single-stranded DNA,ssDNA)免疫标记法检测海马内ssDNA阳性细胞(凋亡细胞)数,并通过透射电镜观察脑超微结构。结果发现次声作用后大鼠学习记忆功能下降、海马ssDNA阳性细胞数明显增多(与正常对照组相比,P<0.05);电镜下可见神经细胞出现较严重缺血、缺氧性改变,并可见不同时期凋亡细胞。依达拉奉干预组大鼠学习记忆成绩与空白对照组相比无差异;海马ssD-NA阳性细胞数较次声对照组明显减少,但仍较正常对照组多,差异均有统计学意义;电镜下细胞变性改变程度较轻。由此得到结论16Hz、130dB次声可引发大鼠的脑损伤、细胞凋亡、记忆功能受损,依达拉奉可明显减轻次声引发的这些损害。
In order to observe the effect of infrasound of 16Hz and 130dB on the memory function of SD rats and the preventive and therapeutic effect of edaravone on its brain damage. The edaravone intervention group began continuous administration (3 mg / kg) three days before infrasound until it stopped after infrasound for 7 days. After infrasound for 7 days, the memory function of rats was evaluated by Y-shaped electric maze, and the number of ssDNA positive cells (apoptotic cells) in the hippocampus was detected by single-stranded DNA (ssDNA) immunohistochemistry. Transmission electron microscopy Brain ultrastructure. The results showed that after infrasound, learning and memory abilities decreased, the number of ssDNA positive cells in hippocampus increased significantly (P <0.05 compared with normal control group), and the changes of ischemia and hypoxia were more obvious under electron microscope Apoptotic cells can be seen at different times. Edaravone intervention group rats learning and memory scores compared with the blank control group was no difference; hippocampus ssD-NA positive cells than the infrasound control group was significantly reduced, but still more than the normal control group, the differences were statistically significant; Electron microscopy changes in the degree of cell degeneration less. It was concluded that 16Hz, 130dB infrasound can cause brain injury in rats, apoptosis, impaired memory, edaravone can significantly reduce these infrasound-induced damage.