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建立了液相色谱串联质谱法测定人血浆中的昂丹司琼(1),并考察其在健康志愿者体内的药动学。以格拉司琼(2)为内标,使用Phenomenex-C18柱,流动相为甲醇∶10 mol/L乙酸铵溶液(45∶55)。质谱采用多反应监测(MRM)模式,正离子检测,监测离子对m/z 294.1→m/z 170.2(1)和m/z 313.4→m/z 138.1(2)。1在0.1~80.0 ng/ml范围内线性关系良好,日内和日间RSD小于8.0%。18名男性健康志愿者口服1盐酸盐片后的主要药动学参数为:c_(max)(51.3±6.5)ng/ml,t_(max)(1.6±0.2)h,t1/2(4.2±0.9)h,AUC_(0→t)(257.6±64.1)ng·h·ml~(-1),AUC_(0→∞)268.0±70.1)ng·h·ml~(-1)。
A liquid chromatographic tandem mass spectrometry was developed for the determination of ondansetron (1) in human plasma and its pharmacokinetics in healthy volunteers. Granisetron (2) was used as the internal standard, and Phenomenex-C18 column was used. The mobile phase was methanol: 10 mol / L ammonium acetate solution (45:55). The mass spectra were monitored by multiple reaction monitoring (MRM) mode with positive ion detection. The ion pair m / z 294.1 → m / z 170.2 (1) and m / z 313.4 → m / z 138.1 (2) were monitored. 1 ranged from 0.1 to 80.0 ng / ml with good RSD of less than 8.0% during the day and day. The main pharmacokinetic parameters of 18 male healthy volunteers after oral administration of 1 hydrochloride tablets were: c max 51.3 ± 6.5 ng / ml, t max 1.6 ± 0.2 h, t1 / 2 4.2 ± 0.9) h, AUC_ (0 → t) (257.6 ± 64.1) ng · h · ml -1, AUC_ (0 → ∞) 268.0 ± 70.1) ng · h · ml -1.