论文部分内容阅读
为观察对二氯苯(P-DCB)致小鼠免疫功能损伤及微量元素的变化,将100只健康成年清洁级昆明小鼠按体重随机分5组,分别为阴性对照(正常空气)组和低(0.64 g/m3)、中(2.56 g/m3)、高(1.28 g/m3)剂量染毒组及阳性对照(以40 mg/kg环磷酰胺腹腔注射染毒,隔天1次)组,每组20只,雌雄各半。静式吸入染毒14 d,每天2 h。测定免疫器官(脾脏和胸腺)重量、免疫功能(足跖增厚程度和半数溶血值)及血中微量元素锌、锰、镁、铜的含量。结果显示,与阴性对照组比较,高剂量组胸腺和脾脏系数、足跖增厚程度、血中锌、铜含量以及中、高剂量组半数溶血值均较低,差异有统计学意义(P<0.05);且随着对二氯苯染毒剂量的升高,小鼠胸腺和脾脏系数、足跖增厚程度和半数溶血值以及血中锌、铜含量均呈下降趋势。各剂量组血中Mg、Mn含量与阴性对照组比较,差异均无统计学意义。提示对二氯苯抑制小鼠的细胞免疫和体液免疫功能,可能与体内微量元素Zn、Cu的含量降低有关。
In order to observe the immunological damage and trace element changes in mice induced by P-DCB, 100 healthy adult Kunming mice were randomly divided into 5 groups according to body weight, which were negative control (normal air) group and (0.64 g / m 3), medium (2.56 g / m 3) and high (1.28 g / m 3) dose groups and the positive control group (intraperitoneal injection of cyclophosphamide at 40 mg / kg once every other day) , Each 20, male and female half. Static inhalation exposure 14 d, 2 h daily. The weight of immune organs (spleen and thymus), immune function (degree of plantar thickening and half hemolytic value) and contents of trace elements zinc, manganese, magnesium and copper in blood were measured. The results showed that compared with the negative control group, the thymus and spleen coefficient, the degree of plantar thickening, the contents of zinc and copper in the high dose group and the half hemolytic value in the middle and high dose group were lower, the difference was statistically significant (P < 0.05). With the increase of the dose of p-dichlorobenzene, the thymus and spleen coefficient, the degree of plantar thickening, half hemolytic value and the contents of zinc and copper in the blood of mice decreased. There was no significant difference in the contents of Mg and Mn in each dose group compared with the negative control group. Tip dichloro benzene inhibited cellular and humoral immune function in mice may be related to the body’s trace elements Zn, Cu content decreased.