本发明涉及使用肟脱水酶从肟生物催化地产生腈的新方法和具有肟脱水酶活性的新突变体及其在用于生物催化产生腈的方法,如特别地用于从柠檬醛肟、橙花醛肟、香叶醛肟或香茅醛肟产生柠檬醛腈、橙花醛腈、香叶醛腈或香茅腈的方法中的用途; The present invention relates to a novel process for the biocatalytic production of nitriles from oximes using oxime dehydratases and to novel mutants having oxime dehydratase activity and their use in the biocatalytic production of nitriles such as those specifically used for the production of nitriles from citral oxime, The use of aldoxime, geranial oxime or citronium oxime to produce citral nitrones, nerolidin, geranonylnitrile or citronellyl nitrile;