论文部分内容阅读
材料与方法山桃仁〔Prunus davidiana(Carr.)Franch〕系延安野生山桃之种仁。按1977年版中国药典制成1:1煎剂,置冰箱备用。实验用延安本地产红公鸡,体重相差不超过0.1kg,随机分为给药组与对照组,给药组公鸡每天以山桃仁煎剂灌胃两次,每次2.57ml/kg,对照组灌以同量蒸馏水,连灌3—4天后进行实验。实验前用5%戊巴比妥钠肌肉注射50—70mg/kg,然后进行全麻,固定,快速插气管插管,随时清除气管分泌物,以保证呼吸道通畅。分离一侧颈静脉与另一侧颈总动脉。在三段聚乙烯管的中段与硅胶管交接的接口处,即在靠动脉一端的两管接口处,放入一根用分析天平精确称重的,长5cm 的4号手术丝线,线的一端是在硅胶管中游离,另一端是在硅胶管与聚乙烯管接口钳夹着,外面只露2mm 即可,以免随动脉血流冲走阻塞细管。将肝素的生理盐水溶液(每ml50单位)用注射器灌满聚乙烯管管腔,小心将聚乙烯管的一头尖端(靠近丝线游离的一头)插入已剪好小V 形口内,用丝线固定结扎好,再补充管内肝素溶液并迅速用血管夹夹住管壁,然后用动脉夹夹住对侧动脉近心端,
MATERIALS AND METHODS Prunus davidiana (Carr.) Franch was the kernel of Yanan wild peach. According to the 1977 edition of the Chinese Pharmacopoeia made 1: 1 decoction, set the refrigerator spare. Experimental use Yan’an local red cocks, body weight difference of not more than 0.1kg, were randomly divided into the treatment group and the control group, the treatment group cock daily Shantao Yan decoction two times, each 2.57ml / kg, the control group of irrigation With the same amount of distilled water, even 3-4 days after the experiment. Before the experiment with 5% pentobarbital sodium intramuscular injection of 50-70mg / kg, and then general anesthesia, fixed, rapid intubation, tracheal clearance at any time to ensure that the airway patency. One side of the jugular vein and the other side of the common carotid artery. In the middle of the three sections of polyethylene pipe and silicone tube interface at the junction of the two ends of the artery by one end of the interface, into an analytical balance accurately weighed, the length of 5cm surgical wire No. 4, one end of the line Silicone tube is the free, the other end is in the silicone tube and polyethylene pipe clamp, outside only exposed 2mm, so as to avoid blocking the blood flow with blocking blood vessels. The normal saline solution of heparin (50 units per ml) was filled with polyethylene syringe tube cavity, carefully a polyethylene pipe tip (near the free end of the wire) has been cut into a small V-shaped mouth has been cut, with a fixed wire ligation , Then add intrahepatic heparin solution and quickly clamp the wall with the vascular clamp, and then use the artery clamp the proximal end of the contralateral artery,