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一、材料与方法 1.以包含有野生型人类U1小核核糖核酸基因序列的质粒pBSIISK+（2.96kb）为模板进行PCR反应:引物1:5’-ACTCCG GAT GTG CTG ACC CCTGCG A TTG CTG TCTGA TGAGTC CGT GAG CAC GAA ACG
I. Materials and Methods 1. PCR was performed using plasmid pBSIISK + (2.96 kb) containing the wild-type human U1 small ribonucleic acid gene sequence as a template: Primer 1: 5’-ACTCCG GAT GTG CTG ACC CCTGCG A TTG CTG TCTGA TGAGTC CGT GAG CAC GAA ACG