论文部分内容阅读
观察干扰素治疗过程中ＨＥＶＤＮＡ量的变化。方法与结果对－组ｅ抗原阴性ＨＢＶＤＮＡ阳性用干扰素治疗的慢性乙型肝炎患者，用分枝链ＤＮＡ（ｂＤＮＡ）信号扩增试验、聚合酶链反应及膜杂交试验检测血清中ＨＢＶＤＮＡ，其检出率分别为７６．５％、１００％和４７．１％用ｂＤＮＡ信号扩增试验观察了这组患者在干扰素治疗过程中ＤＮＡ含量的变化，发现８２．４％的患者治疗后ＤＮＡ降至低于检测水平，其中３２％的患者出现反跳；６１．８％的思者血清ＤＮＡ含量变化与ＡＬＴ反应相一致。结论ｂＤＮＡ信号扩增试验可定量检测病毒核酸，是考核抗病毒药物疗效的较好方法。
Observe the change of the amount of HEVDNA during interferon treatment. Methods and Results HBeAg-positive HBVDNA-positive patients with chronic hepatitis B treated with interferon were tested for HBVDNA in serum by DNA strand amplification (BDNA) signal amplification, polymerase chain reaction and membrane hybridization The rates of DNA content changes during the interferon treatment were observed by bDNA signal amplification assay in 76.5%, 100% and 47.1% of the patients, respectively. The results showed that 82.4% Less than the level of detection, 32% of patients rebound; 61.8% of the changes in SZ DNA content consistent with the ALT response. Conclusion The bDNA signal amplification assay can detect viral nucleic acid quantitatively, which is a good way to evaluate the efficacy of antiviral drugs.