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背景:甲醛作为一种有毒物质可给人体带来严重的损害,同时又缺少有效的防治方法。目的:观察中药三七复方合剂对甲醛熏染大鼠肝细胞增殖的影响。方法:将SD大鼠随机分成对照组、甲醛组和三七治疗组。对照组不做任何处理;甲醛组每天置于静态熏染箱内行甲醛熏染,连续8周;三七治疗组处理同甲醛组,但自第5周起,每日给予三七复方合剂灌胃1次。结果与结论:苏木精-伊红染色结果显示,甲醛熏染8周甲醛组大鼠肝组织肝小叶形态结构紊乱、肝细胞呈现明显的增殖现象,窦间隙缩窄,而三七治疗组肝组织的形态结构明显恢复。免疫组织化学结果显示,甲醛组大鼠肝组织增殖细胞核抗原阳性细胞数明显多于对照组(P<0.01),而三七治疗组与甲醛组相比则明显降低(P<0.01)。结果证实,三七复方合剂能够降低由甲醛诱发的肝组织增殖细胞核抗原的表达,可有效减缓由甲醛诱发的大鼠肝细胞增殖。
Background: Formaldehyde as a toxic substance can cause serious damage to the human body, while lacking an effective control method. Objective: To observe the effect of Panax notoginseng mixture on the proliferation of hepatocytes induced by formaldehyde in rats. Methods: SD rats were randomly divided into control group, formaldehyde group and Panax notoginseng group. The control group did not do any treatment; the formaldehyde group was placed in a static alveolus formaldehyde formaldehyde infection every day for 8 weeks; Panax notoginseng treatment group with the same formaldehyde group, but from the 5th week, the daily given Panax notoginseng mixture 1 times . RESULTS AND CONCLUSION: The results of hematoxylin-eosin staining showed that the hepatic lobule morphology was disorganized in the formaldehyde-treated group for 8 weeks and the hepatocytes showed obvious proliferation phenomenon. The sinus gap was narrowed. However, the hepatic tissue The morphological structure of the obvious recovery. The results of immunohistochemistry showed that the number of proliferating cell nuclear antigen positive cells in the liver of the formaldehyde group was significantly more than that of the control group (P <0.01), while that of the three groups was significantly lower than that of the formaldehyde group (P <0.01). The results confirmed that notoginseng compound mixture can reduce the formaldehyde induced liver tissue proliferating cell nuclear antigen expression, can effectively reduce the formaldehyde-induced rat liver cell proliferation.