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目的:观察银杏内酯B(GKB)对体外分化的神经干细胞(NSC)神经突起生长的影响,并初步探讨其可能的机制。方法:用含不同浓度(20 mg/L、40 mg/L、60 mg/L)GKB的分化培养基培养NSC,在不同时间点测量细胞突起的数量和长度,在第7天时应用免疫荧光染色法检测细胞因子信号转导抑制因子-2(SOCS2)表达。结果:(1)24 h和3 d时各GKB组NSC神经突起长度和数量均较对照组显著增加(P<0.01);40 mg/L和60 mg/L GKB组NSC神经突起数量和平均长度较20 mg/L GKB组显著增加(P<0.01),但两者之间无显著差异。(2)各GKB组和对照组3 d时NSC神经突起长度比24 h时显著增加(P<0.01);对照组3 d时NSC神经突起数量与24 h时无显著差异,而相同GKB组间NSC平均神经突起数量均显著增加(P<0.01)。(3)各GKB组SOCS2免疫反应物平均吸光度分别为1.382、1.578和1.527,均显著高于对照组(1.134,P<0.01);40 mg/L和60 mg/L GKB组SOCS2免疫反应物平均吸光度显著高于20 mg/L GKB组(P<0.01),但40 mg/L GKB组与60 mg/L GKB组无显著差异。结论:GKB能够促进体外分化的NSC神经突起生长,表现为神经突起数量和长度增加;其作用机制可能与SOCS2表达上调有关。
Objective: To observe the effect of ginkgolide B (GKB) on the growth of neural stem cells (NSCs) differentiated in vitro and to explore its possible mechanism. Methods: NSCs were cultured in differentiation media containing GKB at different concentrations (20 mg / L, 40 mg / L, 60 mg / L), the number and length of the cells were measured at different time points, and immunofluorescence staining Method to detect the expression of cytokine signaling inhibitor-2 (SOCS2). Results: (1) The neurite length and number of NSC in GKB group increased significantly at 24 and 3 days (P <0.01), while the number and average length of NSC neurite in 40 and 60 mg / L GKB group Compared with 20 mg / L GKB group increased significantly (P <0.01), but no significant difference between the two. (2) The neurite length of NSC significantly increased in each GKB group and control group at 3 d (P <0.01), while the number of NSC neurites in control group at 3 d was not significantly different from that at 24 h The average number of neurites in NSC increased significantly (P <0.01). (3) The average absorbance of SOCS2 immunoreactant in GKB group was 1.382,1.578 and 1.527, respectively, which were significantly higher than those in control group (1.134, P <0.01). The average SOCS2 immunoreactant in 40 mg / L and 60 mg / L GKB group The absorbance was significantly higher than that of 20 mg / L GKB group (P <0.01), but there was no significant difference between 40 mg / L GKB group and 60 mg / L GKB group. CONCLUSION: GKB can promote the growth of NSC neurons in vitro. The number and length of neurites are increased. Its mechanism may be related to the up-regulation of SOCS2 expression.