论文部分内容阅读
目的:优选火绒草中总黄酮的大孔吸附树脂富集纯化工艺。方法:采用静态吸附/解吸与动态吸附/解吸相结合的方法,用紫外-可见分光光度法测定火绒草中总黄酮含量,筛选最佳大孔吸附树脂,并对其纯化工艺进行优选。结果:确定采用HPD100型大孔吸附树脂对火绒草中总黄酮进行富集纯化,其最佳工艺条件为以2 BV火绒草供试品溶液(生药质量浓度0.1 g·mL-1)为最大上样量,吸附速率为1 BV·h-1,吸附2 h,洗脱流速4 BV·h-1,5 BV水洗脱,4 BV 30%乙醇洗脱,3 BV 50%乙醇洗脱,收集洗脱液,减压浓缩,即得。按上述最佳纯化工艺总黄酮洗脱率>90%。结论:该优选工艺稳定可行,适合工业化生产。
Objective: To optimize the enrichment and purification process of macroporous adsorption resin of total flavonoids in. Methods: The content of total flavonoids in Leonurus chinense was determined by the method of static adsorption / desorption and dynamic adsorption / desorption. The best macroporous resin was screened by UV-Vis spectrophotometry and the purification process was optimized. Results: It was determined that HPD100 macroporous resin was used to enrich and purify the total flavonoids in the flax grass. The optimum conditions were as follows: 2 BV of turpentine (0.1 g · mL-1) The sample volume was 1 BV · h-1, adsorption time was 2 h, elution flow rate was 4 BV · h-1,5 BV, eluted with 4 BV 30% ethanol and eluted with 3 BV 50% ethanol. Eluate, vacuum concentration, that is, too. According to the above optimal purification process total flavonoids elution rate> 90%. Conclusion: The optimal process is stable and feasible, suitable for industrial production.