论文部分内容阅读
目的 检测并分析中国成都汉族群体线粒体 DNA控制区多态性 ,为法医学应用提供基础数据。方法 应用 PCR扩增和直接测序方法检测 10 0个不相关中国成都汉族群体线粒体 DNA控制区中高变区 、 的序列多态性。结果 检测高变区 中 4 0 4个核苷酸和高变区 中 379个核苷酸的序列 ,在 区和 区分别检测到 92和 5 0个变异点 ,与 Anderson标准序列比较 ,共检测到 97种单倍型。偶合概率分别为1.84 %与 1.94 % ,两者合并后为 1.18%。结论 提示线粒体 DNA控制区 DNA序列多态性在法医学领域中具有较高的应用价值。
Objective To detect and analyze the mitochondrial DNA control region polymorphism of Han population in Chengdu, China and provide basic data for forensic application. Methods PCR amplification and direct sequencing were used to detect the polymorphism of hypervariable regions in mitochondrial DNA control region of 100 unrelated Han Chinese population in Chengdu, China. Results Four hundred and forty nucleotides in the hypervariable region and 379 nucleotides in the hypervariable region were detected. 92 and 50 mutation points were detected in the region and region respectively. Compared with Anderson standard sequence, 97 haplotypes. The coupling probability was 1.84% and 1.94%, respectively, and the combined probability was 1.18%. The results suggest that DNA sequence polymorphism in the control region of mitochondrial DNA has high value in forensic medicine.