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为观察不同感染度日本血吸虫病小鼠急、慢性期心脏的病理、超微结构及相关生化代谢的变化 ,采用光镜、电镜对日本血吸虫病小鼠模型的心肌组织的病理变化进行观察 ,同时检测血清心肌酶 AST、CK活性 ,心肌组织超氧化物歧化酶 (SOD)活性及丙二醛 (MDA)含量。结果显示 ,自感染后第 8wk轻、重度感染组心肌组织均可见明显的病理改变。主要表现为心内膜、心肌及心肌血管急性炎性病变及慢性过程中的纤维母细胞增生 ,局部间质纤维化 ,线粒体肿胀 ,肌浆网扩张 ,糖原颗粒增多等变化。在心肌组织内发现有虫卵沉着 ,但未发现成虫。AST活性在第8、18wk,CK活性在第 13、18wk均显著升高。SOD活性先升高 (第 8wk)后降低 (第 13、18wk) ,MDA含量自第 8wk起持续升高。结果提示 ,日本血吸虫感染致宿主心脏损伤是虫卵沉着、氧自由基的继发损伤等因素共同作用的结果。
To observe the changes of pathology, ultrastructure and related biochemical metabolism in the acute and chronic heart of schistosomiasis japonica mice with different infection degrees, the pathological changes of myocardium in the mouse model of schistosomiasis japonica were observed by light microscope and electron microscope. The activities of AST and CK, the activities of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in myocardium were measured. The results showed that since the 8th week after infection, the pathological changes of myocardium were observed in the light and severely infected groups. Mainly for endocardial, myocardial and myocardial vascular acute inflammatory lesions and chronic fibroblast proliferation, local interstitial fibrosis, mitochondria swelling, sarcoplasmic reticulum dilation, increased glycogen granules and other changes. Found in the myocardium of egg deposition, but did not find adults. AST activity in the eighth, 18wk, CK activity in the first 13,18wk were significantly increased. The activity of SOD first increased (8th week) and then decreased (13th and 18th week). MDA content continued to increase from 8th week. The results suggest that the host heart damage caused by Schistosoma japonicum infection is the result of the co-action of egg deposition, secondary damage of oxygen free radicals and other factors.