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目的 :研究苦参碱 (MT)和氧化苦参碱 (OMT)分别对二乙基亚硝胺 (DEN)诱发大鼠肝癌的预防阻断作用。方法 :采用 0 .0 1%的DEN诱发大鼠肝癌 90d ,同时分别腹腔注射MT和OMT注射液 15mg/kg ,停止诱癌及给药处理 30d后 ,处死大鼠 ,观察大鼠肝脏的病理改变、肝表面癌结节数、肝 /体重比和血清中丙氨酸氨基转移酶 (ALT)、γ 谷氨酰转肽酶 (γ GT)、碱性磷酸酶 (ALP)的变化。结果 :MT组和OMT组大鼠的体重明显高于模型组 ,肝表面癌结节数、肝 /体重比和血清ALT、γ GT明显低于模型组 (P<0 0 5) ,而ALP较模型组有所升高。另外 ,OMT组的肝重、肝 /体重比和血清ALP均明显低于MT组(P <0 0 5)。结论 :MT和OMT ,尤其是OMT ,尽管不能完全阻断DEN诱发大鼠肝癌的发生 ,但能保护肝细胞免受损伤 ,延缓DEN诱发大鼠肝癌的形成
Objective: To study the preventive effect of matrine (MT) and oxymatrine (OMT) on diethylnitrosamine (DEN)-induced liver cancer in rats. METHODS: Rat liver cancer was induced with 0.01% DEN for 90 days, while intraperitoneal injection of 15 mg/kg MT and OMT injections were stopped. After induction of cancer treatment and treatment for 30 days, the rats were killed and the liver pathological changes were observed. The number of liver surface cancer nodules, liver/body weight ratio, and serum alanine aminotransferase (ALT), gamma glutamyl transpeptidase (γ GT), and alkaline phosphatase (ALP) changes. Results: The body weights of rats in MT and OMT groups were significantly higher than those in the model group. The number of liver surface nodules, liver/body weight ratio and serum ALT, γ GT were significantly lower than those in the model group (P<0.05). The model group has increased. In addition, liver weight, liver/body weight ratio, and serum ALP were significantly lower in the OMT group than in the MT group (P < 0.05). Conclusion : MT and OMT, especially OMT, can not completely block the occurrence of hepatocellular carcinoma induced by DEN in rats, but it can protect liver cells from injury and delay the formation of liver cancer induced by DEN in rats.