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土壤微生物在森林生态系统中起着重要作用。高通量测序方法的出现为进一步认识土壤微生物提供了契机。本文利用Illumina Miseq高通量测序技术对宝天曼森林土壤的细菌多样性进行了初步研究。结果显示:在31个采样点内,随着采样点增加,检测出不同分类水平的土壤细菌类群也在增多,当采样点达到31个时,检测出的土壤细菌类群达到45门163纲319目495科785属和42,632个OTU;31个土壤样品中所检测出的细菌类群平均有34.2门114.7纲215.2目323.7科446.6属5,924.7个OTU,其中门、纲、目分类水平上的优势类群(所占比例)分别为变形菌门(Proteobacteria)(38.30%)、α-变形菌纲(α-Proteobacteria)(18.08%)、根瘤菌目(Rhizobiales)(10.62%)。这些初步研究结果表明在一定程度上宝天曼森林土壤有较高的细菌多样性水平,为进一步认识森林土壤细菌多样性与植物多样性关系等奠定了基础。
Soil microorganisms play an important role in forest ecosystems. The emergence of high-throughput sequencing methods provides an opportunity to further understand soil microorganisms. In this paper, we used the Illumina Miseq high-throughput sequencing technique to study the bacterial diversity of forest soil in Baotianman. The results showed that with the increase of sampling points, the number of soil bacterial groups detected at different classification levels increased at 31 sampling points. When the number of sampling points reached 31, the number of soil bacterial groups detected reached 45 163 classes and 319 orders 4985 families and 6832 OTUs. The average number of bacterial groups detected in 31 soil samples was 34.2, 114.7, 215.2, 323.7, 446.6, and 5,924.7 OTUs, respectively. Among them, the dominant groups Proteobacteria (38.30%), α-Proteobacteria (18.08%) and Rhizobiales (10.62%), respectively. The results of these preliminary studies indicate that to some extent, Baotianman forest soil has a higher level of bacterial diversity, which lays the foundation for further understanding of the relationship between forest soil bacterial diversity and plant diversity.