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为明确不同浓度NH4HCO3、Cu SO4·5H2O和Fe Cl3·6H2O对南方根结线虫Meloidogyne incognita 2龄幼虫活性的影响,在盆栽条件下将化合物处理后的2龄幼虫接种番茄,通过根染色和根系冰冻切片法观察2龄幼虫的发育状况和根系内巨细胞的形成,并测定了番茄根系各生理指标的变化。结果表明,50mmol/L NH4HCO3、1.4mmol/L Cu SO4·5H2O和1.4 mmol/L Fe Cl3·6H2O处理南方根结线虫后,番茄根系内2龄、3龄及4龄幼虫数量均显著小于对照;根系内丙二醛、可溶性糖和游离氨基酸的含量均显著低于对照,根系活力显著增强;经NH4HCO3处理后,根系内巨细胞形成缓慢,14 d时出现巨型细胞空洞现象。3种化合物处理对南方根结线虫生长发育的抑制作用显著,处理后的线虫对番茄植株致病力减弱,表现为NH4HCO3抑制作用最强,Cu SO4·5H2O次之,Fe Cl3·6H2O最弱。
To clarify the effect of different concentrations of NH4HCO3, CuSO4.5H2O and FeCl3.6H2O on the activity of 2nd instar larvae of Meloidogyne incognita, the 2nd instar larvae were inoculated with the compound under potted conditions and rooted and rooted frozen The development of 2nd instar larvae and the formation of giant cells in root system were observed by slicing method. The changes of each physiological index of tomato root system were also determined. The results showed that after treatment with 50 mmol / L NH4HCO3, 1.4 mmol / L CuSO4 · 5H2O and 1.4 mmol / L FeCl3 · 6H2O, the numbers of 2nd, 3rd and 4th instar larvae in tomato root system were significantly less than those in control. The contents of malondialdehyde, soluble sugar and free amino acids in root system were significantly lower than those in the control, and the root activity was significantly increased. After treatment with NH4HCO3, the formation of giant cells in the root system was slow, and giant cell cavitation appeared on the 14th day. The three compounds had significant inhibitory effects on the growth and development of Meloidogyne incognita. The treated nematodes attenuated the pathogenicity of tomato plants, showing the strongest inhibitory effect of NH4HCO3, followed by CuSO4.5H2O and the weakest of FeCl3.6H2O.