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采用基因枪法、电脉冲法、农杆菌介导法和浸泡法将外源基因质粒pZFX2导入小麦细胞中 ,经卡那霉素筛选 ,获得了抗性的愈伤组织和再生植株。通过此项试验 ,研究了不同方法的转化效果 ,摸索了适宜的小麦基因转化条件
The exogenous gene plasmid pZFX2 was introduced into wheat cells by particle bombardment, electroporation, Agrobacterium-mediated and soaking methods. After selection by kanamycin, resistant callus and regenerated plants were obtained. Through this experiment, the transformation effects of different methods were studied, and the suitable wheat gene transformation conditions