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建立了液相色谱-二极管阵列检测器(HPLC-DAD)测定猪肾中三聚氰胺含量的分析方法. 利用乙腈、 水和HCl混合提取液或三氯乙酸(1+99)溶液涡旋、超声萃取样品中的三聚氰胺, 将提取液离心、过MCX阳离子交换柱后采用HPLC-DAD进行检测. 在0.5～100 mg/L范围内具有良好的线性关系, RSD＜5% (n=7);检测限(S/N=10)为1 mg/kg. 应用所建立的方法测定了猪肾、饲料、 猪尿、猪肉等样品中的三聚氰胺含量.“,”An analytical method for the determination of melamine in pig′s kidney extraction. The extracted solution was centrifuged and separated on a MCX cationic solid-phase extraction column, and then oletermined by HPLC-DAD. The good linearity in the range of 0.5～100 mg/kg was obtained. The relative standard deviation (RSD) of peak area was less than 5% (n=7). The limit of detection for melamine was 1 mg/kg. This method could be applied to the analysis of melamine in pig′s kidney, feed, swine urine and pork, which provided a reference method for the detection of melamine.