近期上海口岸连续多次从进境的玫瑰鲜切花中检出我国检疫性有害生物李属坏死环斑病毒(Prunus necrotic ringspot virus,PNRSV),通过DAS-ELISA、RT-PCR、SYBR Green I实时荧光RT-PCR和序列分析等4种方法分别检测和复验该病毒,结果均为阳性,由此确定从进境玫瑰鲜切花中检出了PNRSV。同时本文分别检测玫瑰叶片、花瓣、茎干和花萼,结果均带有PNRSV,确定该病毒系统侵染玫瑰。 In recent days, the Shanghai port has repeatedly detected Prunus necrotic ringspot virus (PNRSV) from our country's rose cut flowers. The real-time fluorescence of SYBR Green I was detected by DAS-ELISA, RT-PCR, RT-PCR and sequence analysis of the four methods were tested and re-examination of the virus, the results were positive, thus determining the fresh rose cut flowers from the detected PNRSV. At the same time, we detected rose leaves, petals, stems and calyxes, all with PNRSV, and confirmed that the virus infected rose.