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目的研究银杏黄酮(GBE)对卡铂(CBDCA)肾毒性的防护作用,并探讨其可能机制。方法灌胃给予大鼠GBE后腹腔内注射CBDCA,检测肾脏系数、血清尿素氮(BUN)及尿N-乙酰-β-D氨基葡萄糖苷酶(NAG)、血还原型谷胱甘肽(GSH)与肾皮质丙二醛(MDA)及线粒体谷胱甘肽过氧化酶(GSH-Px)及尿与肾皮质铂含量,观察GBE的防护作用的剂量依赖关系和经时过程。结果250、500和750mg/kgGBE预处理后第5天,CBDCA所致大鼠肾脏系数、NAG活性和BUN含量增高均不同程度减轻;500mg/kg组GBE预处理的效果最明显,该组肾脏系数、NAG活性和BUN含量分别为(0.79±0.12)g/100g、(15.86±3.28)U/gCre和(9.27±3.77)mmol/L,而CBDCA组这3项指标分别为(0.96±0.22)g/100g、(23.58±5.45)U/gCre和(31.08±15.00)mmol/L,上述各项指标两组间的差异有显著性(P<0.05或0.01)。GBE预处理可抑制CBDCA引起的MDA形成增高,GSH含量和GSH-Px活性下降,并能降低CBDCA所致大鼠肾皮质铂含量增高,促进铂经尿排泄。结论GBE有明显预防CBDCA的肾毒性,其部分机制为抗氧化作用,还可能与促进铂清除有关。
Objective To study the protective effect of ginkgetin (GBE) on renal toxicity of carboplatin (CBDCA) and to explore its possible mechanism. Methods The rats were injected intraperitoneally with CBDCA after intragastric administration of GBE, and the renal coefficient, serum urea nitrogen (BUN), urinary N-acetyl-β-D glucosaminidase (NAG), glutathione (GSH) And renal cortical malondialdehyde (MDA) and mitochondrial glutathione peroxidase (GSH-Px) and urinary and renal cortical platinum content, the protective effect of GBE dose-dependent and time-dependent process. Results On the 5th day after pretreatment with 250, 500 and 750 mg / kgGBE, the increase of renal coefficient, NAG activity and BUN in CBDCA rats were all alleviated to some extent. The effect of GBE pretreatment on 500 mg / kg group was the most obvious. The renal coefficient , NAG activity and BUN content were (0.79 ± 0.12) g / 100g, (15.86 ± 3.28) U / gCre and (9.27 ± 3.77) mmol / L respectively, while those in CBDCA group were (0.96 ± 0.22) g /100g, (23.58 ± 5.45) U / gCre and (31.08 ± 15.00) mmol / L respectively. There was a significant difference between the two groups (P <0.05 or 0.01). GBE pretreatment could inhibit the increase of MDA formation, GSH content and GSH-Px activity induced by CBDCA, and decrease the platinum content in renal cortex of CBDCA rats and promote the excretion of platinum in the urine. Conclusion GBE can significantly prevent nephrotoxicity of CBDCA. Some of its mechanisms are anti-oxidative and may be related to the promotion of platinum clearance.