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研究他莫昔芬(Tamoxifen,TAM)联合γ射线辐照对脑胶质瘤细胞PKCα蛋白表达的影响,初步探讨TAM降低辐射抗性的机制。采用Western-Blot法检测PKCα和G1期调控蛋白CylinD1的表达;碱性单细胞凝胶电泳法检测DNA链断裂。结果提示,TAM联合γ射线辐照可诱导PKCα蛋白和CyclinD1蛋白表达量降低,增加单纯照射对DNA的损伤并抑制其修复。结果提示,TAM联合γ射线辐照能够增强TAM或辐照单独处理对PKCα蛋白表达的下调、诱导CyclinD1低表达和抑制DNA损伤修复,与TAM降低SHG-44细胞的辐射抗性有关。
To study the effect of Tamoxifen (TAM) combined with γ-ray irradiation on the protein expression of PKCα in glioma cells and to explore the mechanism of TAM in reducing radiation resistance. The expression of CylinD1, a regulator of PKCα and G1 phase, was detected by Western-Blot. DNA strand breaks were detected by alkaline single-cell gel electrophoresis. The results suggest that combination of TAM and γ-ray irradiation can reduce the expression of PKCα protein and CyclinD1 protein, increase DNA damage and inhibit the repair of pure DNA. The results suggest that TAM combined with γ-ray irradiation can enhance the downregulation of PKCα protein expression induced by TAM or irradiation, induce the low expression of CyclinD1 and inhibit the DNA damage repair, which is related to the decrease of radiation resistance of SHG-44 cells by TAM.