采用单因素试验和响应面分析法优化灵芪菌质多糖固态发酵条件。优化后发酵条件为:采用药性固体发酵培养基,初始pH6.5,接种量10.16ml/100g,发酵温度30.65℃,相对湿度59.54%,发酵周期28d。利用此优化条件进行灵芪菌质多糖发酵,灵芪菌质多糖含量可达9.12±0.09mg/g,较优化前8.06±0.07 mg/g提高了13.15%,为开展其药效学研究及产业化研发提供理论试验依据。 Optimization of Solid Fermentation Conditions of Lingqi polysaccharide by single factor experiment and response surface analysis. The optimized fermentation conditions were as follows: the initial pH was 6.5, the inoculation amount was 10.16ml / 100g, the fermentation temperature was 30.65 ℃, the relative humidity was 59.54% and the fermentation period was 28 days. Using this optimization conditions, the polysaccharide from Lingqi polysaccharides was up to 9.12 ± 0.09mg / g, which was increased by 13.15% compared with 8.06 ± 0.07 mg / g before optimization. To carry out its pharmacodynamics and industry R & D to provide theoretical basis for testing.