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目的:研究解毒通络保肾胶囊对链脲菌素糖尿病(DM)大鼠肾组织中单核细胞趋化蛋白-1(MCP-1)表达的影响。方法:以链脲菌素诱导的糖尿病大鼠为动物模型,采用免疫组化及RT-PCR技术检测各组肾小球中MCP-1的表达水平。结果:解毒通络保肾胶囊能明显改善糖尿病大鼠一般状态,降低尿蛋白,改善肾功能,减少肾小球中MCP-1蛋白及基因表达。结论:解毒通络保肾胶囊的肾保护作用可能与减少肾小球中激活的MCP-1表达,减轻肾组织中单核巨噬细胞(ED-1)浸润有关。
Objective: To study the effect of Jiedu Tongluobaoshen capsule on the expression of monocyte chemoattractant protein-1 (MCP-1) in kidney of streptozotocin-induced diabetic rats. Methods: Streptozotocin-induced diabetic rats were used as an animal model. Immunohistochemistry and RT-PCR were used to detect the expression of MCP-1 in glomeruli. Results: Jie Duo Tong Luo Bao Shen Capsule can significantly improve the general state of diabetic rats, reduce urinary protein, improve renal function, and reduce glomerular MCP-1 protein and gene expression. Conclusion: The renal protective effect of Jie Duo Tong Luo Bao Shen Capsule may be related to the decrease of the expression of activated MCP-1 in the glomerulus and the reduction of mononuclear macrophage (ED-1) infiltration in renal tissue.