作者用同位素标记的合成寡核苷酸探针进行Northern印迹分析、原位杂交,并用免疫组织化学法对丁型肝炎病毒(HDV)在旱獭肝脏和肝外组织(脾、肾和外周血淋巴细胞)中的HDV RNA复制和HDAg表达进行了全面的研究.8只感染了旱獭肝炎病毒(WHV)的旱獭中7只重叠感染HDV,1只为对照.用于原位杂交的反义链探针是~35S标记 The authors performed Northern blot analysis using isotopically labeled synthetic oligonucleotide probes, in situ hybridization, and immunohistochemical detection of hepatitis D virus (HDV) in the liver and extrahepatic tissues of mullet (spleen, kidney and peripheral blood lymphocytes ) HDV RNA replication and HDAg expression were investigated in a comprehensive study.7 Eight of the eight chickens infected with the woodchuck hepatitis virus (WHV) were superinfected with HDV and one was used as a control. The antisense strand probe used for in situ hybridization Is ~ 35S mark