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目的使用前脑特异性敲除早老素-1(PS-1FB-KO)的小鼠模型,观察饮食对其体重的影响以及表没食子儿茶素没食子酸酯(EGCG)对该小鼠体重调节的作用。方法将2月龄体重相近的PS-1FB-KO和同窝对照(CON)雄性小鼠各分为2组分别给予高脂和普通膳食。连续喂养8周后再将高脂喂食组各分为3组进行为期1周的以下处理:(1)恢复普通饲料;(2)继续给予高脂饲料同时腹腔注射EGCG;(3)继续给予高脂饲料同时腹腔注射PBS。同期连续监测小鼠的体重和血糖值。结果实验前8周,PS-1FB-KO高脂膳食喂养组小鼠的体重增长率小于CON组。实验第9周,PS-1FB-KO与CON高脂膳食喂养同时腹腔注射EGCG的体重减低率大于同基因型注射PBS组。PS-1FB-KO组与CON组的血糖在各时期并无显著性差异。结论 PS-1FB-KO使小鼠的体重调节功能产生障碍,并且这种差异并不是由外周代谢差异引起的。EGCG能够降低高脂诱导肥胖小鼠的体重,并且其潜在的中枢神经保护作用,帮助PS-1FB-KO的小鼠减轻肥胖。
Objective To investigate the effects of diet on body weight and the effects of epigallocatechin-3-gallate (EGCG) on the body weight of mice using PS-1FB-KO mouse model. effect. Methods Two-month-old male PS-1FB-KO mice and the same littermate control (CON) were divided into two groups and given a high fat and normal diet respectively. After continuous feeding for 8 weeks, the high-fat diet group was divided into 3 groups for one week following treatment: (1) normal feed recovery; (2) continuous high fat diet with intraperitoneal injection of EGCG; (3) continuous high Lipid feed was injected intraperitoneally into PBS. Simultaneous monitoring of body weight and blood glucose levels in mice. Results Eight weeks before the experiment, the body weight gain of PS-1FB-KO high-fat diet group was less than that of CON group. The weight loss rate of PSC-1FB-KO and CON peritoneal injection of EGCG at the 9th week of the experiment was higher than that of the same-genotype PBS group. There was no significant difference in blood glucose between PS-1FB-KO group and CON group at each time point. CONCLUSIONS: PS-1FB-KO impaired the regulation of body weight in mice, and the difference was not caused by peripheral metabolic differences. EGCG reduced the body weight of obese mice fed high fat diet and its potential central neuroprotective effect helped PS-1FB-KO mice to reduce obesity.