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目的研究2009年中国甲型H1N1流感病毒的进化特性和变异情况,为甲型H1N1流感的监控和防治提供科学依据。方法利用ClustaX进行序列比对,以Bioedit软件进行序列同源性分析,使用Mega 4.0软件采用邻位相连算法构建进化树,对2009年登录在Genbank数据库中的中国甲型H1N1流感病毒的HA、NA、M蛋白的氨基酸序列进行系统进化分析。结果中国2009年甲型H1N1流感病毒分离株与美国分离株[A/California/04/2009(H1N1)]具有很近的亲缘关系,不同省份分离株之间的HA、NA、M蛋白相互交叉渗透,但同时各个省份间暴发的H1N1流感病毒的HA蛋白又具有一定的聚集性。香港分离株[A/Hong Kong/2369/2009(H1N1)]和上海分离株[A/Shanghai/LWS1/2009(H1N1)]产生了对达菲的耐药性,其他毒株仍然敏感;上海分离株[A/Shanghai/LWS1/2009(H1N1)]对金刚烷胺类药物敏感,其余毒株均对其耐药。结论本研究表明,中国2009年甲型H1N1流感病毒不仅具有很强的保守性,同时又具有一定的区域特异性,毒株相对稳定,没有出现大的变异。
Objective To study the evolutionary characteristics and variation of 2009 H1N1 influenza virus in China and provide a scientific basis for the surveillance and prevention of Influenza A (H1N1). Methods Sequence alignment was performed using ClustaX software. Bioedit software was used to analyze the sequence homology. The phylogenetic tree was constructed using the software Mega 4.0 using the ortho-linked method. The HA and NA of the Chinese Influenza A (H1N1) virus registered in the Genbank database in 2009 , M protein amino acid sequence phylogenetic analysis. Results The 2009 H1N1 influenza virus isolates in China were closely related to the U.S. isolates [A / California / 04/2009 (H1N1)]. The HA, NA and M proteins of different provinces cross-infiltrated each other , But at the same time the HA protein of the H1N1 influenza virus that broke out in various provinces again has certain aggregation. Resistant to Tamiflu was produced in Hong Kong isolates [A / Hong Kong / 2369/2009 (H1N1)] and Shanghai isolates [A / Shanghai / LWS1 / 2009 (H1N1)]; other strains were still sensitive; Strain [A / Shanghai / LWS1 / 2009 (H1N1)] was sensitive to amantadine and other strains were resistant to it. Conclusion This study shows that China 2009 Influenza A (H1N1) virus is not only highly conserved, but also has some regional specificity, the strain is relatively stable, there is no major variation.