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目的初步研究维吾尔药材地锦草有效部位体外抗真菌作用,研究地锦草有效部位对红色毛癣菌琥珀酸脱氢酶活性的影响,探讨其抗真菌作用机制。方法地锦草醇提物经大孔吸附树脂分离得6个洗脱部位,分别为部位一、部位二、部位三、部位四、部位五及部位六;采用微量液基稀释法测定各部位体外最低抑菌浓度(MIC)值,确定其最有效部位和敏感菌株;药物干预毛癣菌后分离菌体,按试剂盒要求处理样品,利用硫酸甲酯吩嗪(PMS)反应法,测定地锦草有效部位对红色毛癣菌琥珀酸脱氢酶活性的作用。结果地锦草各洗脱部位中,部位六的抗真菌活性最为显著,其对红色毛癣菌(T.rubrum)的平均MIC为149μg/mL,对石膏样毛癣菌(T.mentagrophytes)的平均MIC为149μg/mL,对其他皮肤癣菌平均MIC为192μg/mL,对4种念珠菌的平均MIC均为416μg/mL。不同浓度的地锦草有效部位均可降低红色毛癣菌琥珀酸脱氢酶活性(P<0.01)。结论地锦草不同提取部位对试验菌株的生长均具有抑制作用,其中部位六尤为显著,对浅部致病真菌有较强的抑制作用,定为有效部位。地锦草有效部位抗真菌机制可能与抑制琥珀酸脱氢酶的活性有关。
OBJECTIVE To study the antifungal effect of the active part of Ustilagoae officinalis in vitro and to study the effect of the active part of Ustilago Fortuensis on the activity of succinic dehydrogenase of Trichophyton rubrum and to explore its antifungal mechanism. Methods The extract of the ethanol extract of P. philoxeroides was separated into six fractions by macroporous adsorption resin, which were part one, part two, part three, part four, part five and part six, respectively. The minimum inhibitory concentration (MIC) was determined to determine the most effective part and the sensitive strain. After the drug interfered with Trichophyton mentagrophytes, the cells were separated and treated according to the requirements of the kit. Effect of the Available Parts of Grass on Succinate Dehydrogenase Activity of Trichophyton. Results The antifungal activity of Part 6 was the most significant among the fractions of P. euphratica. The average MIC against T.rubrum was 149 μg / mL, and the inhibitory effect against T. mentagrophytes The average MIC was 149 μg / mL, the average MIC for other dermatophytes was 192 μg / mL, and the average MIC for all 4 Candida species was 416 μg / mL. Different concentrations of Euphorbiaceae effective sites can reduce the Trichophyton rubrum activity of succinate dehydrogenase (P <0.01). Conclusion Different extract fractions of F. gramineus have inhibitory effects on the growth of test strains. Among them, Part 6 is especially significant, which has a strong inhibitory effect on superficial pathogenic fungi and is regarded as an effective site. Antifungal mechanism of effective parts of Euonymus fortunei may be related to the inhibition of succinate dehydrogenase activity.