论文部分内容阅读
目的 :检测 P2 1WAF 1 及 P16蛋白在乳腺癌中的表达情况 ,探讨两种蛋白表达与肿瘤大小、临床分期、腋淋巴结转移、术后 5 a生存率等预后指标之间的关系。方法 :应用 P2 1WAF 1单克隆及 P16多克隆抗体 ,对 86例乳腺癌石蜡包埋标本进行免疫组化 SP法的检测。结果 :P2 1WAF 1 的检出率为 5 1.2 %,P16检出率为 45 .3%。P2 1W AF 1 的阳性信号定位于胞核 ,为棕黄色细颗粒状均匀分布。P16主要定位于胞核 ,亦可见胞浆着色。P2 1WAF 1及 P16的表达与组织学类型、临床分期、肿瘤大小及 5 a生存率显著相关 ,但与腋淋巴结转移无显著相关性。结论 :乳腺癌的生物学性状与 P2 1WAF 1 及 P16蛋白的表达有关。
Objective : To detect the expression of P2 1WAF 1 and P16 in breast cancer and to explore the relationship between the expression of these two proteins and the prognostic indicators such as tumor size, clinical stage, axillary lymph node metastasis, and postoperative 5-year survival rate. Methods : P2 1WAF 1 monoclonal antibody and P16 polyclonal antibody were used to detect paraffin-embedded specimens of 86 breast cancer specimens by immunohistochemical SP method. Results: The detection rate of P2 1WAF 1 was 5 1.2 %, and the detection rate of P16 was 45.3 %. The positive signal of P2 1W AF 1 was located in the nucleus and was evenly distributed in brown and yellow fine particles. P16 is mainly located in the nucleus and cytoplasm staining is also seen. The expression of P2 1WAF 1 and P16 was significantly associated with histological type, clinical stage, tumor size, and 5-year survival rate, but was not significantly associated with axillary lymph node metastasis. Conclusion: The biological characteristics of breast cancer are related to the expression of P2 1WAF 1 and P16 proteins.