目的:建立复方异丙安替比林片含量测定的HPLC方法。方法:色谱柱为Zorbax SB-C18(4．6×150 mm,5μm),流动相为乙腈-水-冰醋酸(68:32:1．0),检测波长为280 nm,流速为1．0 ml·min-1。结果:异丙安替比林和氯唑沙宗线性范围分别为5．4-27．1μg·ml-1(r=0．999 8),5．3-26．7μg·ml-1(r=0．999 8),检测限分别为0．20 ng和0．22 ng,平均回收率分别为99．6％,RSD0．4％和99．9％RSD0．6％(n=9)。结论:该方法简便、结果准确,可同时测定复方制剂中2种成分。 Objective: To establish a HPLC method for the determination of compound isoproterenol tablets. METHODS: The mobile phase consisted of Zorbax SB-C18 (4.6 × 150 mm, 5 μm) with a mobile phase of acetonitrile-water-glacial acetic acid (68:32: 1.0) at a detection wavelength of 280 nm and a flow rate of 1.0 ml · min-1. RESULTS: The linear ranges of isoproterenol and chlorzoxazone were 5.4-27.1 μg · ml-1 (r = 0.999 8), 5.3-26.7 μg · ml-1 (r = 0.999 8). The detection limits were 0.20 ng and 0.22 ng, respectively. The average recovery was 99.6%, RSD 0.4% and 99.9% RSD 0.6%, respectively (n = 9). Conclusion: The method is simple and accurate. The two components in compound preparations can be determined simultaneously.