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目的用多种技术方法研究探讨柯萨奇B组病毒(CBV)与习惯性流产的病毒病因。方法采用ELISA、RT-PCR和病毒分离对86例习惯性流产和40例人工流产妇女进行了CBV-IgM抗体、病毒RNA和病毒粒子检测。结果检测结果表明习惯性流产和人工流产两组妇女柯萨奇B组病毒IgM阳性率分别为87.2%和35.0%,血淋巴细胞CBVRNA阳性率则为53.5%和17.5%;而胎盘组织中病毒RNA阳性率各为59.3%和17.5%,胎盘组织中病毒分离阳性率在两组妇女各为41.9%和6.9%。其各项指标经!2检验,两组均有显著性差异(P<0.01)。结论柯萨奇B组病毒可能是习惯性流产的发生因素之一。
Objective To explore the etiological factors of virus in Coxsackie B virus (CBV) and habitual abortion by various techniques. Methods CBV-IgM antibody, viral RNA and virion were detected in 86 cases of habitual abortion and 40 cases of induced abortion by ELISA, RT-PCR and virus isolation. Results The results showed that the positive rate of IgM in Coxsackie B group was 87.2% and 35.0% respectively in both habitual abortion and abortion women and 53.5% and 17.5% in hemoblasts. However, the viral RNA The positive rates were 59.3% and 17.5% respectively. The positive rates of virus isolation in placental tissues were 41.9% and 6.9% in both groups. The indicators by! 2 test, the two groups were significantly different (P <0.01). Conclusion Coxsackie B virus may be one of the factors of habitual abortion.