目的　体外扩增恶性疟原虫海南分离株的已糖转运体基因 (pfHT1) ,构建恶性疟原虫HT1基因真核融合表达载体并检测其表达情况 ,为用于DNA免疫作准备。方法　特定PCR引物的设计 ,扩增 ,基因组DNA提取 ,连接 ,鉴定 ,细胞培养 ,脂质体转染 ,荧光显微镜观察。结果　从恶性疟原虫海南分离株基因组DNA中扩增特异的编码 pfHT1的基因序列 ,片断大小为 15 16bp ,经酶切鉴定证实成功构建 pEGFP -HT1融合表达质粒 ,将其转染体外培养的细胞后 ,可见融合蛋白表达产生的明亮的绿色荧光。结论　成功构建 pEGFP -HT1融合表达质粒 ,并实现在细胞内的表达。为进行动物体内的DNA免疫打下了基础。 Objective To amplify pfHT1 gene of Plasmodium falciparum isolates from Hainan Province in vitro and construct eukaryotic fusion expression vector of Plasmodium falciparum HT1 gene for detection of its expression. Methods Design, amplification, genomic DNA extraction, ligation, identification, cell culture, lipofection, and fluorescence microscopy of specific PCR primers. Results The pfHT1-encoding gene was amplified from the genomic DNA of Plasmodium falciparum isolates from Hainan. The fragment was 15 16 bp. The recombinant plasmid pEGFP -HT1 was successfully constructed and transfected into cells cultured in vitro , Showing the bright green fluorescence produced by the fusion protein expression. Conclusion The recombinant plasmid pEGFP -HT1 was successfully constructed and expressed in cells. Laid the foundation for the DNA immunization in animals.