论文部分内容阅读
目的对1例输入性疑似疟疾患者的血样进行实验室确诊。方法首先制备血涂片,吉姆萨染色后镜检疟原虫;其次对血样进行RDT检测;最后利用疟原虫属特异性(通用型)和4种疟原虫种特异性的巢式PCR和多重PCR检测方法,对该血样进行分子生物学检测及分型。结合分子生物学检测结果,再对血片进行镜检复核。结果初次镜检结果为间日疟原虫,RDT结果为阴性。巢式PCR检测结果,仅扩增出预期大小约800bp的卵形疟条带;多重PCR(Pf/Pv)检测无特异性条带产生。重新对薄血膜复核镜检,改判为卵形疟原虫。结论综合巢式PCR、多重PCR、RDT和镜检等检测结果,确诊该患者为卵形疟原虫感染。
Objective To confirm the laboratory diagnosis of blood samples from 1 suspected malaria patient. Methods The blood smear was prepared firstly, the plasmodium was detected after Giemsa staining, then the blood samples were tested by RDT. Finally, the specificity of the parasite genus and the four kinds of Plasmodium species were detected by nested PCR and multiplex PCR Methods, the blood samples for molecular biology detection and typing. Combined with molecular biology test results, and then the blood film for microscopic examination. The results of the first microscopic examination of Plasmodium vivax, RDT results were negative. The results of nested PCR only amplified the expected size of about 800bp oval-shaped malaria bands; multiple PCR (Pf / Pv) detection of non-specific bands. Re-examination of the thin blood film microscopy, re-classified as ova-type Plasmodium. Conclusion The results of nested PCR, multiplex PCR, RDT and microscopic examination confirmed that the patient was Plasmodium ovale infection.