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目的 探讨双环醇对刀豆蛋白A (ConA)引起小鼠肝脏细胞核和DNA损伤的保护作用。方法 用ConA诱发小鼠肝损伤模型 ,观察双环醇对血清转氨酶 (ALT) ,肝细胞核DNA片断化、肝细胞核内DNA对CuSO4 邻啡罗啉 维生素C H2 O2 系统损伤的敏感性以及肝脏中H2 O2 含量的作用。结果 双环醇 5 0、15 0mg/kg灌胃能保护ConA引起的小鼠肝损伤 ,降低血清中ALT水平 ,P值分别小于 0 0 5、0 0 1;减轻肝脏细胞核和DNA的损伤 ,荧光测定DNA片断化数值降低 (P <0 0 5 ,P <0 0 1) ,DNA条带出现百分率由ConA组的 10 0 %降低至 2 5 % ;防止肝细胞核DNA对CuSO4 邻啡罗啉 维生素C H2 O2 系统损伤的敏感性的降低 ,并能阻抑肝脏H2 O2 的升高 (双环醇 15 0mg/kg组 :P <0 0 5 )。双环醇在体外加药 (终浓度分别为 :4,16 ,6 4× 10 -7mol/L)也能直接保护CuSO4 邻啡罗啉 维生素C H2 O2系统对纯品DNA的损伤。结论 双环醇对ConA引起的小鼠肝细胞核DNA损伤具有明显的保护作用。
Objective To investigate the protective effect of bicyclol on the nuclear and DNA damage induced by ConA in mice liver. Methods ConA-induced mouse liver injury model was used to observe the sensitivity of bicyclic alcohol to serum aminotransferase (ALT), nuclear DNA fragmentation of hepatocytes, the sensitivity of DNA in liver cells to CuSO4 o-morin vitamin C H2 O2 system injury and H2O2 The role of content. Results Bicyclol 500 mg / kg and 150 mg / kg were able to protect ConA-induced liver injury and reduce the level of ALT in serum, P values were less than 0 0 5,0 0 1 respectively; DNA fragmentation decreased (P <0.05, P <0.01), and the percentage of DNA bands decreased from 100% in ConA group to 25% O2 system lessen the sensitivity of the injury, and can inhibit the liver H2O2 increased (bicyclic alcohol 150mg / kg group: P <0 05). Bicyclol in vitro (final concentrations: 4,16,6 × 10 -7 mol / L, respectively) could also directly protect the pure DNA of CuSO4-vicinal morpholine vitamin C H2 O2 system. Conclusion Bicyclol has a significant protective effect on ConA-induced DNA damage in liver cells of mice.