Improvement of Properties of Pseudomonas sp.Lipase in Resolution of 2-Octanol

来源 :Chemical Research in Chinese Universities | 被引量 : 0次 | 上传用户:xiaojianlan
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A novel preparation method was developed to form a surfactant-lipase complex for the resolution of 2-octanol in a solvent-free system. The E value was improved from 6.64 to 120.2. The lipase modified by the anionic surfactant possessed a low solubility in the solvent-free system, which was beneficial to the recovery and the repeated usage of the lipase. The surfactant-lipase complex maintained a high enantioselectivity after five cycles of usage. The effect of water on both the activity and the enantioselectivity of the lipase has also been investigated; the direct addition of a salt hydrate pair of Na 4P 2O 7·H 2O and Na 4P 2O 7 can dramatically activate the modified enzyme. A novel preparation method was developed to form surfactant-lipase complex for the resolution of 2-octanol in a solvent-free system. The E value was improved from 6.64 to 120.2. The lipase modified by the anionic surfactant possessed a low solubility in the The surfactant-lipase complex maintained a high enantioselectivity after five cycles of usage. The effect of water on both the activity and the enantioselectivity of the lipase has also the direct addition of a salt hydrate pair of Na 4P 2O 7 · H 2O and Na 4P 2O 7 can dramatically activate the modified enzyme.
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