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目的观察西北寒燥证证候模型小鼠下丘脑-垂体-肾上腺轴的功能状态,初步诠释新疆高发疾病的共性生物学基础。方法通过人工气候箱模拟新疆寒燥环境,并作用于小鼠,21天后眼球取血处死,取下丘脑、垂体、肾上腺分别在光镜和电镜下观察其组织形态学和细胞超微结构的改变,并测定其外周血中促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、皮质酮(CORT)、β-内啡肽(β-EP)含量的变化。结果与空白组比较,西北寒燥证证候模型组小鼠下丘脑、垂体、肾上腺存在器官组织形态学和细胞超微结构的改变,血清中ACTH含量升高(P<0.01),CRH、CORT、β-EP含量未见明显差异(P>0.05)。结论西北寒燥证证候模型小鼠下丘脑-垂体-肾上腺轴存在结构和功能状态的紊乱,这些可能是寒燥环境导致机体产生不同于其他地区人群病理生理状态,进而一些疾病在新疆高发的微观机制之一。
Objective To observe the functional status of the hypothalamus-pituitary-adrenal axis in mice model of syndrome of cold and cold syndrome in Northwest China, and tentatively explain the common biological basis of the high incidence of disease in Xinjiang. Methods The artificial climate chamber was used to simulate the frigid environment in Xinjiang and the mice were sacrificed. After 21 days, the eyeballs were sacrificed and the hypothalamus, pituitary and adrenal glands were removed for histopathological and ultrastructural changes under light and electron microscopes respectively The levels of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosterone (CORT) and β-endorphin (β-EP) in peripheral blood were measured. Results Compared with the blank group, the histopathological changes and ultrastructure of the hypothalamus, pituitary and adrenal gland in the mouse model of cold syndrome in the Northwest Cold and Dry Type showed that the serum levels of ACTH were increased (P <0.01), CRH and CORT , Β-EP content no significant difference (P> 0.05). Conclusions The syndromes of hypothalamus-pituitary-adrenal axis in mice with syndrome of cold syndrome in Northwest Cold and Dry Type syndromes may have disorders in their structure and function. These may be caused by cold and dry environment, which may lead to the pathophysiological state different from that in other areas. Some diseases are more frequent in Xinjiang. One of the micro-mechanisms.