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目的探讨血管紧张素Ⅱ受体(AT1R和AT2R)基因多态性与肾上腺腺瘤(APA)发病之间是否存在关联性。方法应用聚合酶链式反应-限制性片段长度多态性方法(PCR-RFLP)检测100例健康对照者(对照组)及85例APA患者(观察组)的AT1R基因1166A/C和AT2R基因1675A/G多态性。结果观察组AT1R基因1166A/C基因型(AA、AC、CC)及等位基因(A、C)频率分布与对照组比较差异无统计学意义,观察组AT2R基因1675A/G基因型(AA、AG、GG)与等位基因(A、G)频率分布与对照组比较差异有统计学意义(P<0.05),G等位基因频率较A等位基因频率显著升高。结论 AT2R 1675A/G的基因突变可能与APA发病存在显著关联。
Objective To investigate the relationship between the polymorphism of angiotensin Ⅱ receptor (AT1R and AT2R) and the incidence of adrenal adenoma (APA). Methods The AT1R gene 1166A / C and AT2R gene 1675A of 100 healthy controls (control group) and 85 APA patients (observation group) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) / G polymorphism. Results There was no significant difference in the frequency distribution of AT1R gene 1166A / C genotypes (AA, AC, CC) and alleles (A, C) between the observation group and the control group. The AT2R gene 1675A / AG, GG) and allele (A, G) were significantly different from those in control group (P <0.05). The frequency of G allele was significantly higher than that of A allele. Conclusion The gene mutation of AT2R 1675A / G may be associated with the pathogenesis of APA.