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目的:观察平喘Ⅰ号对呼吸道合胞病毒(RSV)感染引起BALB/c小鼠哮喘模型血清中肿瘤坏死因子-a(TNF-a)、内皮素-1(ET-1)含量的影响,探讨其治疗哮喘发作的作用机制。方法:72只BALB/c小鼠随机分成6组,随机分为正常对照组、模型对照组、地塞米松组、平喘Ι号低、中、高剂量组,每组12只。予RSV致敏,除正常对照组和模型对照组用等量生理盐水外,其余各组用相应药物干预。末次激发48h后取血清采用ELISA法测定各组TNF-a和ET-1含量。结果:与正常对照组比较,哮喘模型组小鼠血清TNF-a、ET-1含量明显增高(P<0.01)。与模型对照组比较,平喘I号各剂量组和地塞米松组均可降低哮喘小鼠血清中TNF-a和ET-1水平(P<0.01或P<0.05),其中平喘I号高剂量组与地塞米松组水平接近。结论:平喘Ⅰ号能降低哮喘小鼠血清TNF-a、ET-1含量,并具有量效关系。由此推测,通过降低血清中TNF-a和ET-1水平,从而减轻气道炎症反应和气道组织的重构,是平喘Ⅰ号治疗哮喘的作用机制之一。
Objective: To observe the effect of Pingchuan I on the levels of TNF-a and ET-1 in asthmatic BALB / c mice infected with respiratory syncytial virus (RSV) To explore its mechanism of action in the treatment of asthma attacks. Methods: Seventy-two BALB / c mice were randomly divided into 6 groups, which were randomly divided into normal control group, model control group, dexamethasone group and low and middle dose of Pingchuan I, 12 rats in each group. To RSV sensitization, in addition to the normal control group and model control group with the same amount of saline, the other groups with the appropriate drug intervention. After the last 48h, serum was taken and the contents of TNF-a and ET-1 in each group were determined by ELISA. Results: Compared with the normal control group, the levels of serum TNF-a and ET-1 in asthma model group were significantly increased (P <0.01). Compared with the model control group, the levels of TNF-a and ET-1 in serum of asthmatic mice decreased significantly (P <0.01 or P <0.05) Dose group and dexamethasone group level close. Conclusion: Pingchuan I can reduce the level of serum TNF-a and ET-1 in asthmatic mice and has dose-response relationship. It is speculated that by reducing the serum levels of TNF-a and ET-1, thereby reducing airway inflammation and airway remodeling, asthma asthma is one of the mechanisms of action.