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目的探讨右丙亚胺(Dexrazoxane,DEX)对颅脑损伤(traumatic brainin jury,TBI)的大鼠脑组织水和离子含量的影响。方法选择SD大鼠36只,计算机编号随机抽取方法分成正常对照组(n=6)、假手术组(n=10)、损伤组(n=10)和DEX治疗组(n=10),应用液压冲击方法制作TBI模型。DEX组在制作模型后即刻给予DEX,3d后将大鼠处死观察脑组织基底节区域的铁染色情况,以及该区域的水及钠钾离子的变化情况。结果损伤组大鼠脑基底节区域在TBI后3d铁染色明显增强,铁染色阳性细胞比例明显上升,水及钠离子含量也明显上升,而钾离子则没有明显变化。应用DEX治疗后的TBI大鼠铁阳性细胞比例,水及钠离子含量都有明显的下降,显著低于损伤组(P<0.05)。结论DEX可以降低TBI后的水及钠离子含量,这直接影响到脑组织的水肿,其机制可能与降低脑组织的铁沉积有关。
Objective To investigate the effect of dexrazoxane (DEX) on water and ion content in brain tissue of rats with traumatic brain in jury (TBI). Methods Thirty - six SD rats were selected and randomly divided into normal control group (n = 6), sham operation group (n = 10), injury group (n = 10) and DEX treatment group Hydraulic Impact Method to Make TBI Model. The DEX group was given DEX immediately after the model was made. After 3 days, the rats were sacrificed to observe the iron staining in the basal ganglia area of the brain and the change of water, sodium and potassium in the area. Results The basal ganglion area in TBI group was significantly increased after 3 days of TBI, the percentage of iron-stained cells was significantly increased, and the content of water and sodium ions also increased obviously. However, there was no obvious change of potassium ions. After treatment with DEX, the proportion of iron-positive cells, water and sodium in TBI rats decreased significantly, which was significantly lower than that in injury group (P <0.05). Conclusion DEX can reduce the water and sodium ion content after TBI, which directly affects the edema of brain tissue, which may be related to the reduction of iron deposition in brain tissue.