论文部分内容阅读
目的 探讨原钒酸钠 (SOV)对心肌细胞的毒性作用及其机制。方法 采用循环灌流的方法急性分离成年豚鼠心肌细胞 ,使其在原钒酸钠浓度为 1 ,1 0 ,1 0 0 μmol·L- 1 ,1mmol·L- 1 作用后 ,分别检测 30 ,60 ,1 2 0 ,1 80min 4个时相心肌细胞保存液中LDH、CK含量以及心肌细胞蛋白含量和Na+ ,K+ ATP酶活性 ,检测心肌细胞凋亡率和细胞活力 ;电镜下观察死亡和凋亡细胞的细胞器和细胞核形态学变化。结果 在原钒酸钠 1 0 0 μmol·L- 1 和 1mmol·L- 1 两个组的各时相保存液中LDH、CK以及心肌细胞中蛋白含量增加 ;心肌细胞中Na+ ,K+ ATP酶活性和细胞活力降低 ,与对照组相比差异有显著性 (P <0 0 1 )。流式细胞仪显示 :在 1 80min时 1 0 0 μmol·L- 1 和 1mmol·L- 1 两组的细胞凋亡率与对照组相比增加 (P <0 0 5)。结论 在原钒酸钠浓度高于 1 0 0μmol·L- 1 且作用时间在 30min以上时 ,心肌细胞出现不可逆性损害
Objective To investigate the toxic effects of sodium orthovanadate (SOV) on cardiomyocytes and its mechanism. Methods The cardiac myocytes of adult guinea pigs were acutely isolated by the method of perfused circulation. After the concentration of sodium orthovanadate was 1, 100, 100 micromol x L (-1) and 1 mmol·L -1, respectively, 30, 60, 1 The levels of LDH and CK, the protein content of myocardial cells and the activities of Na +, K + -ATPase in cardiomyocytes preservation solution at 2 0, 1 80 min were measured. The apoptosis rate and the viability of cardiomyocytes were detected by electron microscopy. Organelles and nuclear morphological changes. Results The protein contents of LDH, CK and cardiomyocytes were increased in each phase preservation solution of sodium orthovanadate 100 μmol·L -1 and 1 mmol·L -1. The activities of Na +, K + ATPase and Cell viability decreased compared with the control group, the difference was significant (P <0.01). Flow cytometry showed that the rate of apoptosis in the groups of 100 μmol·L-1 and 1 mmol·L-1 at 180 min was higher than that of the control group (P <0.05). Conclusion When the concentration of sodium orthovanadate is higher than 100 μmol·L -1 and the action time is above 30 min, irreversible damage occurs to cardiomyocytes