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目的:分析血管内皮生长因子165b(VEGF165b)在子痫前期(PE)患者血清中的表达,探讨孕早期外周血VEGF165b作为预测PE发生的生物学指标的临床意义。方法:酶联免疫吸附试验(ELISA)和竞争性酶免疫分析(cEIA)分别检测47例PE患者和42例无高血压正常孕妇、40例未怀孕正常妇女血清中VEGF165b、VEGF的含量。ROC曲线分析孕12周VEGF165b预测PE发生的灵敏度。结果:孕12周,正常孕妇组血清VEGF165b含量[(4.49±1.57)ng.ml-1]与未怀孕组[(0.42±0.18)ng.ml-1]相比显著升高(P<0.01),发展成PE患者的外周血VEGF165b含量[(1.19±0.50)ng.ml-1]与正常孕妇组相比显著降低(P<0.01);ROC曲线显示孕12周VEGF165b预测PE发生具有较高的灵敏度(89.8%)。结论:孕早期母体外周血VEGF165b显著降低可预测PE发生。
OBJECTIVE: To analyze the expression of vascular endothelial growth factor 165b (VEGF165b) in sera of patients with preeclampsia (PE) and to explore the clinical significance of peripheral blood VEGF165b in early pregnancy as a biomarker to predict the occurrence of PE. Methods: Serum levels of VEGF165b and VEGF in 47 PE patients and 42 non-hypertensive normal pregnant women and 40 non-pregnant normal women were detected by enzyme linked immunosorbent assay (ELISA) and competitive enzyme immunoassay (cEIA) respectively. ROC curve analysis of the first 12 weeks of pregnancy VEGF165b predicts the sensitivity of PE. Results: Serum levels of VEGF165b in normal pregnant women at 12 weeks pregnant group were significantly higher than those in non-pregnant women [(0.42 ± 0.18) ng.ml-1] [(4.49 ± 1.57) ng.ml-1] (P <0.01). The VEGF165b level in the peripheral blood of patients with PE was significantly lower than that of the normal pregnant women [(1.19 ± 0.50) ng.ml-1] Sensitivity (89.8%). Conclusion: The significantly lower VEGF165b in the maternal peripheral blood can predict the occurrence of PE in the first trimester of pregnancy.