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目的 研究人体骨骼肌失神经支配后成肌调节因子Myogenin和MRF4蛋白的表达变化并了解其再生状态。方法 取 2 0例人体失神经后不同时间段的骨骼肌与 3例正常骨骼肌标本 ,HE染色和透射电镜观察肌细胞核变化 ,抗Myogenin和MRF4多克隆抗体免疫组织化学ABC法染色 ,统计阳性染色细胞核数 ,免疫荧光双重标记染色定性观察Myogenin和MRF4蛋白表达方式。结果 抗Myogenin和MRF4抗体阳性染色细胞核数在人体骨骼肌失神经支配时即开始升高并在 1年内维持在较高水平 ,1年后快速下降 ,至失神经 2年几乎消失。肌细胞核内移现象在失神经 1年内常见且部分居中的细胞核免疫组化染色阳性。结论 骨骼肌失神经支配 1年内 ,核内移可作为肌纤维再生的一个标志 ;Myogenin蛋白表达检测可以作为不可逆肌萎缩的指标 ;周围神经损伤后 ,应在 1年内尽早修复。
Objective To study the expression changes of Myogenin and MRF4 after denervation in human skeletal muscle and to understand their regeneration status. Methods Twenty skeletal muscles and three normal skeletal muscle samples from different periods of human denervation were collected. HE staining and transmission electron microscopy were used to observe the changes of myocyte nuclear. Anti-Myogenin and MRF4 polyclonal antibodies were stained immunohistochemically for ABC The number of nuclei and immunofluorescence double staining were used to qualitatively observe the expression of Myogenin and MRF4 protein. Results The number of nuclei stained positive for anti-Myogenin and MRF4 antibody began to increase when the denervated human skeletal muscle denervated and maintained at a high level within 1 year. The number of nuclei rapidly decreased after 1 year and almost disappeared after 2 years of denervation. Muscle cell nuclear shift phenomenon in the absence of nerve within 1 year common and partially central nuclear immunohistochemical staining. Conclusions Within one year, skeletal muscle denervation can be used as a marker of myofibrillar regeneration. Myogenin protein expression can be used as an indicator of irreversible muscle atrophy. Repaired peripheral nerve injury should be repaired within 1 year as soon as possible.