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目的:建立高效液相色谱法测定大叶金花草中牡荆素含量。方法:采用Waters Symmetry C18(250 mm×4.6 mm,5 μm)色谱柱,流动相为乙腈-0.1%冰乙酸溶液(27∶73),流速为1.0 ml·min-1,检测波长为339 nm,柱温为30℃。结果:牡荆素浓度在10.03~90.27 μg·ml-1范围内与峰面积的线性关系良好(r=0.9999),平均回收率为100.48%,RSD为0.98%。结论:本分析方法准确、灵敏、重现性好,可用于大叶金花草中牡荆素的含量测定。
Objective: To establish a HPLC method for the determination of vitexin in S. macrophylla. METHODS: The mobile phase was acetonitrile-0.1% glacial acetic acid (27:73) at a flow rate of 1.0 ml · min-1 on a Waters Symmetry C18 column (250 mm × 4.6 mm, 5 μm) The column temperature was 30 ° C. Results: The linear relationship between the concentration of vitexin and peak area in the range of 10.03 ~ 90.27 μg · ml-1 was good (r = 0.9999). The average recovery was 100.48% and the RSD was 0.98%. Conclusion: The method is accurate, sensitive and reproducible. It can be used to determine the content of Vitexin in S. auricula.