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目的探讨Pax2与WT1在紫癜性肾炎(HSPN)肾组织中的表达及其两者间的关系。方法选取2004年7月至2005年12月在中南大学湘雅二医院住院的HSPN患儿37例为试验组,肾病综合征患儿(微小病变型)10例为对照组。分别对肾小球和肾小管间质的病理改变程度进行半定量评分,免疫组化检测Pax2与WT1的表达,分析肾脏Pax2、WT1表达与肾脏病理积分及其两者之间的关系。结果(1)HSPN患儿肾组织局部Pax2的表达:Ⅱ级1.14±0.38;Ⅲa级1.71±0.47;Ⅲb级2.58±0.51;Ⅳ级3.00±0.00;对照组0.80±0.45。HSPN患儿与对照组之间和HSPN患儿肾脏病理改变各级间,Pax2的表达差异均有统计学意义(P<0.005),随着肾脏病理级别的加重,Pax2的表达增加。(2)HSPN患儿肾组织局部WT1的表达:Ⅱ级2.78±0.38;Ⅲa级1.86±0.53;Ⅲb级1.50±0.52;Ⅳ级1.50±0.58;对照组2.60±0.54。HSPN患儿与对照组之间和HSPN患儿肾脏病理改变多级间,WT1的表达差异有统计学意义(P<0.05),肾脏病理级别的加重,WT1的表达增加。结论HSPN患儿中存在PaX2的重新表达和WT1的表达减少,且随着肾脏病理加重Pax2的表达增加,WT1的表达减少。
Objective To investigate the expression of Pax2 and WT1 in renal tissue of Purpura nephritis (HSPN) and their relationship. Methods From July 2004 to December 2005, 37 HSPN children hospitalized in Xiangya Second Hospital of Central South University were selected as experimental group and 10 children with nephrotic syndrome (minimal change type) as control group. The degree of pathological changes of glomeruli and tubulointerstitial were evaluated semi-quantitatively. The expressions of Pax2 and WT1 were detected by immunohistochemistry. The relationship between Pax2, WT1 expression and renal pathological scores were analyzed. Results (1) The expression of Pax2 in renal tissues of HSPN children was 1.14 ± 0.38 in grade Ⅱ, 1.71 ± 0.47 in grade Ⅲa, 2.58 ± 0.51 in grade Ⅲb, 3.00 ± 0.00 in grade Ⅳ and 0.80 ± 0.45 in control group. There were significant differences in Pax2 expression between HSPN children and control group and HSPN children (P <0.005). The expression of Pax2 increased with the severity of kidney pathological changes. (2) The expression of WT1 in renal tissues of HSPN patients was 2.78 ± 0.38 in grade Ⅱ, 1.86 ± 0.53 in grade Ⅲa, 1.50 ± 0.52 in grade Ⅲb, 1.50 ± 0.58 in grade Ⅳ and 2.60 ± 0.54 in control group. There was a significant difference in the expression of WT1 between children with HSPN and control group and between HSPN children (P <0.05). The pathological grade of kidney increased and the expression of WT1 increased. Conclusions The expression of Pax2 and WT1 in HSPN patients are decreased, and the expression of Pax2 decreases with the increase of Pax2 in renal pathology.