Butcher(1973)和Jensen(1979)提出体外培养恶性疟原虫可以马血清代替人血清,Sax和Reickmann(1980)曾成功地使用兔血清代替人血清,以后Ifedeba(1980)及Siddiqui(1981)又发现小牛血清加入(月示)蛋白胨可以代替人血清,但这些改进仍均需逐渐减少人血清浓度而相应地增加代用品浓度使疟原虫逐步适应,因而需几个月时间。本文报导以10%绵羊血清代替人血清培养恶性疟原虫一步即可到达适应生长。 Butcher (1973) and Jensen (1979) proposed that P. falciparum could be cultured with horse serum instead of human serum in vitro. Sax and Reickmann (1980) successfully used rabbit serum instead of human serum. Later, Ifedeba (1980) and Siddiqui (1981) Adding bovine serum (peptone) to peptone can replace human serum, but these improvements still require a gradual reduction of human serum concentrations and a corresponding increase in the concentration of surrogates to allow malaria parasite to gradually adapt, thus requiring several months. This article reports that 10% sheep serum instead of human serum culture of Plasmodium falciparum one step to adapt to the growth.