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目的对于北京家族菌株占绝大多数的感染人群,评价多位点可变数量串联重复序列(variable number tandem repeats,VNTR)分析(multiple loci VNTR analysis,MLVA)中不同位点组合在结核分枝杆菌(Mycobacterium tuberculosis,MTB)基因分型研究中的应用。并以IS6110限制性片段长度多态性(restriction fragment length polymorphism,RFLP)为参照,筛选有效位点。方法分别采用IS6110-RFLP、间隔区寡核苷酸分型(Spoligotyping)及MLVA不同位点组合对北京海淀区收集的MTB临床分离株进行基因分型研究,比较3种方法及MLVA不同位点组合的分型效果。结果 45株MTB分离株中86.7%为北京家族菌株,Spoligotyping和结核分枝杆菌散在分布重复单位(mycobacterial interpersed repetitive units,MIRU)-12系列的HGI(Hunter-Gaston Index)值分别为0.4313和0.8700,将45株MTB菌株分为10个和23个基因型。VNTR-9系列和IS6110-RFLP的分型结果一致,HGI值较高,为0.9980,将45株MTB菌株分为43个基因型。结论北京家族结核分枝杆菌在北京海淀区呈高水平流行。对于北京地区北京家族菌株占绝大多数的感染人群,VNTR-9系列MLVA是较为简便和高分辨率的分型方法 ,其分辨率能够达到MTB基因分型“金标准”IS6110-RFLP水平。
Objective To assess the association of different loci in multiple loci VNTR analysis (MLVA) in multi-site infectious population of Mycobacterium tuberculosis (Mycobacterium tuberculosis, MTB) genotyping applications. The IS6110 restriction fragment length polymorphism (RFLP) was used as a reference to screen the effective sites. Methods The genotypes of MTB clinical isolates collected from Beijing Haidian district were genotyped by using IS6110-RFLP, Spoligotyping and MLVA loci respectively. Three methods were compared with those of MLVA The type of effect. Results 86.7% of 45 MTB isolates belonged to Beijing family strains. The HGI (Hunter-Gaston Index) values of Spoligotyping and Mycobacterium interpersed repetitive units (MIRU) -12 series were 0.4313 and 0.8700, 45 MTB strains were divided into 10 and 23 genotypes. The typing results of VNTR-9 series and IS6110-RFLP were consistent, with high HGI values of 0.9980 and 45 strains of MTB divided into 43 genotypes. Conclusion Mycobacterium tuberculosis in Beijing showed a high prevalence in Haidian District, Beijing. VNTR-9 series MLVA is a simple and high-resolution typing method for the majority of infected families in Beijing in Beijing, and its resolution can reach the level of MTB genotyping, “gold standard” IS6110-RFLP .