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目的探讨高迁移率蛋白A2(HMGA2)在不同宫颈病变组织中的表达及其临床意义,了解其与宫颈人乳头瘤病毒(HPV)感染的关系。方法免疫组织化学法检测无锡市第三人民医院2005至2007年30例正常宫颈组织、19例低级别上皮内瘤样病变宫颈组织、16例高级别上皮内瘤样病变宫颈组织、13例宫颈鳞癌组织中HMGA2蛋白的表达,原位杂交检测各组织中HPV感染的情况,分析HMGA2蛋白表达与HPV感染在宫颈病变组织中的相关性。结果正常宫颈组织、CINⅠ级、CINⅡ~Ⅲ级和宫颈浸润性鳞癌组织中,HMGA2蛋白的阳性表达率分别为26.67%、21.05%、68.75%和76.92%。CINI与正常宫颈组织阳性表达率比较,差异无统计学意义;CINⅡ~Ⅲ级、宫颈浸润性鳞癌组织与正常宫颈组织阳性表达率比较,差异有统计学意义(P=0.011,P=0.006)。宫颈高级别上皮内瘤样病变和宫颈癌组织具有较高的HPV感染率,HPV感染与否和HMGA2的表达两者之间未发现统计学相关性。结论HMGA2高表达于高级别宫颈上皮内瘤样病变组织和宫颈癌组织,这可能是宫颈病变的一个早期事件。HMGA2的表达与宫颈HPV感染两者没有相关性,HMGA2可能是宫颈恶性转化的内因之一。
Objective To investigate the expression and clinical significance of high mobility group protein A2 (HMGA2) in different cervical lesions and to understand its relationship with human papillomavirus (HPV) infection. Methods Immunohistochemical method was used to detect 30 cases of normal cervical tissue, 19 cases of low grade intraepithelial neoplasia cervical tissue, 16 cases of high grade intraepithelial neoplasia cervical tissue, 13 cases of cervical squamous cell carcinoma of the Third People ’s Hospital of Wuxi City from 2005 to 2007, The expression of HMGA2 protein in cancer tissues was detected by in situ hybridization. The correlation between HMGA2 protein expression and HPV infection in cervical lesions was analyzed. Results The positive rates of HMGA2 protein in normal cervical tissue, CINⅠ, CINⅡ ~ Ⅲ and cervical invasive squamous cell carcinoma were 26.67%, 21.05%, 68.75% and 76.92% respectively. There was no significant difference in the positive expression rates of CINI and normal cervical tissues between the two groups (P = 0.011, P = 0.006). The positive expression rates of CINⅡ ~ Ⅲ, cervical invasive squamous cell carcinoma and normal cervical tissue were statistically significant . Cervical high-grade intraepithelial neoplasia and cervical cancer tissues have a higher HPV infection rate, HPV infection or not and HMGA2 expression was not found between the two statistically significant. Conclusion HMGA2 is highly expressed in high grade cervical intraepithelial neoplasia and cervical cancer tissues, which may be an early event of cervical lesions. There is no correlation between HMGA2 expression and cervical HPV infection, HMGA2 may be one of the internal causes of cervical malignant transformation.