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目的：探讨内皮拉伤后血管内膜增殖反应的动态变化，和凝血酶对损伤血管增殖反应的影响。 方法：Ｆｏｇａｒｔｙ 导管拉伤大鼠主动脉，不同时期处死，病理观察血管内膜厚度，免疫组织化学观察增殖细胞核抗原在损伤血管的表达；并将离体的血管环在体外加入不同浓度的凝血酶［０ Ｕ／ｍ ｌ（对照组）、１ Ｕ／ｍ ｌ（１ Ｕ凝血酶组）和５ Ｕ／ｍ ｌ（５ Ｕ凝血酶组）］培养１２小时，测定单位重量血管环氚标记胸腺嘧啶脱氧核苷（３Ｈ－ＴｄＲ）的掺入量。 结果：拉伤后血管内膜／中膜面积值于４～６周达到高峰，７～８周增厚的内膜几乎完全消退。增殖细胞核抗原的表达与血管内膜增殖反应基本一致。３Ｈ－ＴｄＲ掺入于术后３日和３ 周出现２个高峰。加入凝血酶１ Ｕ／ｍ ｌ或５ Ｕ／ｍ ｌ，３日的３Ｈ－ＴｄＲ掺入量增加，与０ 日比较有显著性差异Ｐ＜ ０．０５，４周后３Ｈ－ＴｄＲ掺入进入低反应期。 结论：血管损伤后３ 日是细胞分裂最为活跃的时期，对凝血酶促增殖作用也最敏感，４周后为低反应期，因此及早有效抗凝对于控制血管增生和再狭窄有十分重要的意义。
Objective: To investigate the dynamic changes of intimal hyperplasia after endothelial injury and the effects of thrombin on the proliferative responses of injured vessels. Methods: The aorta of rats were injured by Fogarty catheter and sacrificed at different time points. The thickness of intima of the vessel was observed by immunohistochemical method and the expression of proliferating cell nuclear antigen in injured vessels was observed by immunohistochemistry. The isolated vascular rings were treated with different concentrations of thrombin (Control group), 1 U / ml (1 U thrombin group) and 5 U / ml (5 U thrombin group)] for 12 hours, and the unit weight of the blood vessel tritium labeled thymine The incorporation of deoxyribonucleoside (3H-TdR). Results: Intima / media area reached the peak at 4 ~ 6 weeks after injury, and almost completely disappeared at 7 ~ 8 weeks. Proliferating cell nuclear antigen expression and intimal proliferative response are basically the same. 3H-TdR incorporation in the 3 days and 3 weeks after the emergence of two peaks. 3H-TdR incorporation increased on the 3rd with thrombin 1 U / m l or 5 U / m l, and there was significant difference compared with that on the 0th day (P <0.05). 3H-TdR incorporation into the low Reaction period. Conclusion: 3 days after vascular injury is the most active cell division period, the most sensitive to the role of prothrombin proliferation, and 4 weeks after the low response period, so early and effective anticoagulation for the control of vascular proliferation and restenosis have very important significance .